Journal: Development (Cambridge, England)

Article Title: Hemidesmosomes and Notch signaling regulate epidermal differentiation via delamination

doi: 10.1242/dev.205210

Figure Lengend Snippet: Notch signaling regulates integrin-β4 levels and delamination. (A-D) Confocal images of E17 Cre-negative control versus Rosa NICD epidermis (A) and E16 WT littermate versus Rbpj cKO epidermis (C), with single channel images of integrin-β4 on the right, and associated quantification of fluorescence intensity (B,D); n =3 except for WT control in Rbpj cohort ( n =2). (E-G) Images of Notch reporter (NR) transgenic (E) and LUGGIGE NR-transduced epidermis (F), and associated quantification (G). NR shown in green; RFP marks cells transduced with reporter. (H) Quantification of nuclear YAP in Itgb4 4124 epidermis. (I,K) Confocal images of E17 Cre-negative control versus Rosa NICD epidermis (I) and E16 WT littermate versus Rbpj cKO epidermis (K); asterisks indicate dual-positive cells. (J,L) Quantification of dual-positive cells in WT versus mutant. Each dot represents a biological replicate in G,H,J,L, or FOV in B,D, where shapes designate litters. Basement membrane is indicated with cyan dashed line. Scale bars: 25 μm (A,C,E,F,I,K); 10 µm (F, insets). ns, not significant; * P <0.05; **** P <0.0001 ( t -test).

Article Snippet: Transgenic Notch reporter animals were obtained from The Jackson Laboratory [ Tg(Cp-EGFP)25Gaia/J, stock #005854].

Techniques: Negative Control, Fluorescence, Control, Transgenic Assay, Transduction, Mutagenesis, Membrane

Journal: Nature Communications

Article Title: Pericytes are organ-specific regulators of tissue morphogenesis

doi: 10.1038/s41467-026-71643-1

Figure Lengend Snippet: a Labeling of pericytes in the P12 brain cortex via Pdgfrb(BAC)-CreERT2 -mediated, tamoxifen-induced GFP expression (green). Confocal images also show PDGFR (gray), a pericyte marker, and ICAM2, labeling ECs (red). Bottom panels show higher magnification of the yellow dashed boxes in the top row. b Confocal images showing the proximity of GFP+ pericytes (green), Glial fibrillary acidic protein (GFAP)+ astrocytes (magenta) and Allograft inflammatory factor-1 (AIF)+ microglia (gray) in the P12 brain cortex. c Pdgfrb(BAC)-CreERT2 -mediated labeling of pericytes (GFP, green) in the P21 lung. Confocal images also show PDGFRβ (gray) and ICAM2 (red). d Maximum intensity projections showing PDGFRβ+ pericytes (gray) and ICAM2+ ECs (red) in the lung alveolus. Red arrows indicate pulmonary pericytes in the alveolar septum. e Confocal images showing the localization of Prosurfactant Protein C (proSP-C)-expressing type 2 alveolar epithelial cells (green) and receptor for advanced glycation end products (RAGE)-positive type 1 alveolar epithelial cells (gray). f Graphs showing the number (per area/82 × 82 × 6 µm) of pericytes and type 2 alveolar epithelial cells in the septal and alveolar regions of lung alveoli. Data represent mean ± s.e.m. ( n = 5 mice per group); P -values, unpaired two-tailed Student t -test (pericytes) and two-tailed Mann–Whitney test (alveolar epithelial cells). Volcano plots showing the differential expression of genes ( g ) and differentially expressed ligands ( h ) in pericytes from P14 lung versus P12 brain. Average log2-fold change > 0.6, adjusted p -value < 0.05). i Heatmap of differentially expressed ligands (padj < 0.01) in pericytes from postnatal lung versus brain. Pseudobulk DE analysis uses two-sided Wald test + independent filtering as implemented by pyDESeq2 ( g – i ). Source data are provided as a Source Data file.

Article Snippet: In vivo labeling of pericytes was performed by mating Pdgfrb(BAC)-CreERT2 transgenic animals (available as strain #029684 from The Jackson Laboratory) and Rosa26 mT/mG reporter mice .

Techniques: Labeling, Expressing, Marker, Two Tailed Test, MANN-WHITNEY, Quantitative Proteomics